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1x chip buffer  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc 1x chip buffer
    1x Chip Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+buffer/pmc13019507-442-12-15
    Average 86 stars, based on 1 article reviews
    1x chip buffer - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Targeting the ZMYM2-ANXA9 Axis with FLT3 Inhibitor G749 Overcomes Oxaliplatin Resistance in Colorectal Cancer
    Article Snippet: .. The fragmented chromatin was then diluted in ChIP buffer and incubated overnight with anti-HIF1α (CST, 36169, Danvers, MA, USA), anti-HIF2α monoclonal antibody (#59973, CST, Danvers, MA, USA), or anti-ZMYM2 (MABN2263, Millipore, Burlington, MA, USA). .. A normal rabbit IgG (#2729, CST, Danvers, MA, USA) served as a negative control, while histone H3 rabbit monoclonal antibody was used as a positive control.

    Article Title: Proteomic Screening Identifies HNRNPA2B1 as an Epigenetic Repressor of Epstein-Barr Virus Reactivation
    Article Snippet: .. The nuclear pellet was resuspended in 200 μL 1 × ChIP Buffer (Cat. #9003S, Cell Signaling Technology) supplemented with 1 μL of 200 × protease inhibitor cocktail (Cat. #9003S, Cell Signaling Technology) and incubated on ice for 10 minutes. ..

    Article Title: METTL3-mediated SNHG1 m 6 A modification promotes proliferation and migration through transcriptional regulation of WDR74 in osteosarcoma
    Article Snippet: The next day, ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technologies) were added to the samples and incubated for another 4 h at 4°C. .. After 4 h, the beads were harvested and washed three times with 1 × ChIP buffer (Cell Signaling Technologies), followed by a single wash with 1 mL lysis buffer containing 1 × ChIP buffer and 0.5 M NaCl using a magnetic separation rack. .. The chromatin was eluted in a ChIP Elution Buffer (Cell Signaling Technologies) followed by reverse crosslinking at 65°C for at least 4 h. ChIP DNA was treated with 5 g/mL RNase A and 0.2 mg/mL protease K and purified using NucleoSpin Gel and PCR Clean-up (MACHEREY-NAGEL, Düren, Germany).


    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST.
    Article Snippet: .. For ChIP, sonicated chromatin was diluted with 1× ChIP Buffer (Cell Signaling Technology, 7008) at a dilution factor of 1:4. ..

    Article Title: Targeting the ZMYM2-ANXA9 Axis with FLT3 Inhibitor G749 Overcomes Oxaliplatin Resistance in Colorectal Cancer.
    Article Snippet: .. The fragmented chromatin was then diluted in ChIP buffer and incubated overnight with anti-HIF1α (CST, 36169, Danvers, MA, USA), anti-HIF2α monoclonal antibody (#59973, CST, Danvers, MA, USA), or antiZMYM2 (MABN2263, Millipore, Burlington, MA, USA). .. A normal rabbit IgG (#2729, CST, Danvers, MA, USA) served as a negative control, while histone H3 rabbit monoclonal antibody was used as a positive control.

    Article Title: Tceal7 is a BRG1-regulated target of calcineurin signaling that promotes myoblast differentiation
    Article Snippet: .. Nuclei were pelleted, resuspended in 400 μL ChIP buffer (SimpleChIP ® Chromatin IP Buffers, Cell Signaling Technology) supplemented with protease inhibitors, sonicated for 10 min (30 s on/30 s off, medium intensity) using a Bioruptor UCD-200 (Diagenode), and centrifuged at 21,000 × g for 5 min. Fragmented chromatin (200-500 bp) was confirmed by agarose gel electrophoresis. ..

    Incubation:

    Article Title: Targeting the ZMYM2-ANXA9 Axis with FLT3 Inhibitor G749 Overcomes Oxaliplatin Resistance in Colorectal Cancer
    Article Snippet: .. The fragmented chromatin was then diluted in ChIP buffer and incubated overnight with anti-HIF1α (CST, 36169, Danvers, MA, USA), anti-HIF2α monoclonal antibody (#59973, CST, Danvers, MA, USA), or anti-ZMYM2 (MABN2263, Millipore, Burlington, MA, USA). .. A normal rabbit IgG (#2729, CST, Danvers, MA, USA) served as a negative control, while histone H3 rabbit monoclonal antibody was used as a positive control.

    Article Title: Proteomic Screening Identifies HNRNPA2B1 as an Epigenetic Repressor of Epstein-Barr Virus Reactivation
    Article Snippet: .. The nuclear pellet was resuspended in 200 μL 1 × ChIP Buffer (Cat. #9003S, Cell Signaling Technology) supplemented with 1 μL of 200 × protease inhibitor cocktail (Cat. #9003S, Cell Signaling Technology) and incubated on ice for 10 minutes. ..

    Article Title: Targeting the ZMYM2-ANXA9 Axis with FLT3 Inhibitor G749 Overcomes Oxaliplatin Resistance in Colorectal Cancer.
    Article Snippet: .. The fragmented chromatin was then diluted in ChIP buffer and incubated overnight with anti-HIF1α (CST, 36169, Danvers, MA, USA), anti-HIF2α monoclonal antibody (#59973, CST, Danvers, MA, USA), or antiZMYM2 (MABN2263, Millipore, Burlington, MA, USA). .. A normal rabbit IgG (#2729, CST, Danvers, MA, USA) served as a negative control, while histone H3 rabbit monoclonal antibody was used as a positive control.

    Protease Inhibitor:

    Article Title: Proteomic Screening Identifies HNRNPA2B1 as an Epigenetic Repressor of Epstein-Barr Virus Reactivation
    Article Snippet: .. The nuclear pellet was resuspended in 200 μL 1 × ChIP Buffer (Cat. #9003S, Cell Signaling Technology) supplemented with 1 μL of 200 × protease inhibitor cocktail (Cat. #9003S, Cell Signaling Technology) and incubated on ice for 10 minutes. ..

    Lysis:

    Article Title: METTL3-mediated SNHG1 m 6 A modification promotes proliferation and migration through transcriptional regulation of WDR74 in osteosarcoma
    Article Snippet: The next day, ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technologies) were added to the samples and incubated for another 4 h at 4°C. .. After 4 h, the beads were harvested and washed three times with 1 × ChIP buffer (Cell Signaling Technologies), followed by a single wash with 1 mL lysis buffer containing 1 × ChIP buffer and 0.5 M NaCl using a magnetic separation rack. .. The chromatin was eluted in a ChIP Elution Buffer (Cell Signaling Technologies) followed by reverse crosslinking at 65°C for at least 4 h. ChIP DNA was treated with 5 g/mL RNase A and 0.2 mg/mL protease K and purified using NucleoSpin Gel and PCR Clean-up (MACHEREY-NAGEL, Düren, Germany).

    Sonication:

    Article Title: Suppression of HIV-1 transcription and latency reversal via ectopic expression of the viral antisense transcript AST.
    Article Snippet: .. For ChIP, sonicated chromatin was diluted with 1× ChIP Buffer (Cell Signaling Technology, 7008) at a dilution factor of 1:4. ..

    Article Title: Tceal7 is a BRG1-regulated target of calcineurin signaling that promotes myoblast differentiation
    Article Snippet: .. Nuclei were pelleted, resuspended in 400 μL ChIP buffer (SimpleChIP ® Chromatin IP Buffers, Cell Signaling Technology) supplemented with protease inhibitors, sonicated for 10 min (30 s on/30 s off, medium intensity) using a Bioruptor UCD-200 (Diagenode), and centrifuged at 21,000 × g for 5 min. Fragmented chromatin (200-500 bp) was confirmed by agarose gel electrophoresis. ..

    Agarose Gel Electrophoresis:

    Article Title: Tceal7 is a BRG1-regulated target of calcineurin signaling that promotes myoblast differentiation
    Article Snippet: .. Nuclei were pelleted, resuspended in 400 μL ChIP buffer (SimpleChIP ® Chromatin IP Buffers, Cell Signaling Technology) supplemented with protease inhibitors, sonicated for 10 min (30 s on/30 s off, medium intensity) using a Bioruptor UCD-200 (Diagenode), and centrifuged at 21,000 × g for 5 min. Fragmented chromatin (200-500 bp) was confirmed by agarose gel electrophoresis. ..



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